Analysis Note

ControlHUVEC cells.

Application

Immunocytochemistry:
1-10 µg/mL of a previous lot worked in immunocytochemistry.Works best on EDTA or Trypsin lifted endothelial cells.

Immunohistochemistry:
1-10 µg/mL. 4% PFA for 30min RT or ﹤2hrs at 4°C. Block w/ 1%BSA/0.2% tween20/PBS for 30min. Works well in frozen tissue; fixed or unfixed.

Immunoprecipitation:
1-10 µg/mL of a previous lot worked in immunoprecipitation.

ELISA:
1-10 µg/mL of a previous lot worked in ELISA.

FACS Analysis:
1-10 µg/mL of a previous lot worked in FACS.

Optimal working dilutions must be determined by end user.

Anti-MCAM Antibody, clone P1H12 detects level of MCAM & has been published & validated for use in ELISA, FC, IC, IH, IP & WB.

General description

MUC18 antigen (CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MUC18 has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MUC18. The close structural relationship with N-CAM and related molecules suggests that MUC18 may be also a developmentally regulated cell adhesion molecule (Melanoma adhesion molecule or MCAM).

Immunogen

Immunization of mice with HUVEC.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB with 0.25 M NaCl, pH=7.6, with 0.1% sodium azide.

Format: Purified

Quality

Routinely evaluated by Western Blot on HUVEC lysates.

Western Blot Analysis:
1:500 dilution of this lot detected MCAM on 10µg of HUVEC lysates.

Specificity

Detects circulating endothelial cells in human. Expected to cross-react with dog & mouse.

Target description

110 kDa